Immunfluoreszenzoptische Untersuchungen zur experimentellen Glomerulonephritis an Formalin-fixiertem und Paraffin-eingebettetem Gewebe

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Abstract
Immunofluorescence studies are an essential part of the diagnosis in glomerulonephritis. For these studies unfixed, frozen material was used only, on account of intense background staining of formalin-fixed slides. A method developed by Huang (1975) was tested on experimental glomerulonephritis. He succeeded in minimizing the background staining of formalin-fixed liver tissue with pronase-treatment. Our results show no differences in the immunofluorescence of the experimental glomerulonephritis between frozen and paraffin-embedded sections. It seems to be possible therefore to investigate a glomerulonephritis in formalin-fixed material by this method.