West Nile Virus in Southern Portugal, 2004
- 1 December 2005
- journal article
- Published by Mary Ann Liebert Inc in Vector-Borne and Zoonotic Diseases
- Vol. 5 (4) , 410-413
- https://doi.org/10.1089/vbz.2005.5.410
Abstract
West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent surveillance for viral activity within the country.Keywords
This publication has 4 references indexed in Scilit:
- West Nile Virus: An Overview of Its Spread in Europe and the Mediterranean Basin in Contrast to Its Spread in the AmericasEuropean Journal of Clinical Microbiology & Infectious Diseases, 2004
- MEGA2: molecular evolutionary genetics analysis softwareBioinformatics, 2001
- Comparison of Flavivirus Universal Primer Pairs and Development of a Rapid, Highly Sensitive Heminested Reverse Transcription-PCR Assay for Detection of Flaviviruses Targeted to a Conserved Region of the NS5 Gene SequencesJournal of Clinical Microbiology, 2001
- Detection of arboviral RNA directly from mosquito homogenates by reverse-transcription–polymerase chain reactionJournal of Virological Methods, 2001