High Pressure Liquid Chromatographic Separation of 6-Acetoxybenzo(A)pyrene from In Vitro Incubation of 6-Nitrobenzo(A)pyrene
- 1 October 1986
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 9 (13) , 2945-2953
- https://doi.org/10.1080/01483918608074161
Abstract
We carried out studies of the microsomal metabolism of 6-nitrobenzo(a)pyrene (NBaP) to understand why the compound is a marginal carcinogen. Microsomal incubation products were separated by reverse phase high-pressure liquid chromatography. A chromatographic peak with a retention time of 24.8 mins was isolated and examined by ultraviolet, mass and nuclear magnetic resonance spectra. The product was characterized as 6-acetoxy-benzo(a)pyrene. The source of the acetylating agent could be cytosolic.This publication has 14 references indexed in Scilit:
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