THICK-SECTION TECHNIQUE FOR STUDY OF PULMONARY PATHOLOGY

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Abstract
A thick-section technique is described for preparation of lung tissue. The essential steps consist of intratracheal formalin fixation, gelatin embedding and freezing, and sectioning of large blocks at 100 to 500 micra, depending on the character of the tissue. The tissue is then floated in a well slide and stained, dehydrated, and cleared. Mounting in polystyrene plastic (Lustrex) or other material is accomplished on the slides viewed by stereomicroscopy. This technique is especially helpful in the study of the lung, an organ normally containing air spaces. It allows the observation of the arrangement of the capillary bed, the alveolar pores, the elastic framework, and other anatomic structures. It has the advantage of relative simplicity and can be used in conjunction with other pathologic techniques, such as paper section or routine histo logic thin sections. Thick section study of the lung with stereo-microscopy is an indispensable tool in the understanding of lung patho logy.

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