Novel rpoB Mutations Conferring Rifampin Resistance on Bacillus subtilis : Global Effects on Growth, Competence, Sporulation, and Germination
Open Access
- 15 April 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 186 (8) , 2481-2486
- https://doi.org/10.1128/jb.186.8.2481-2486.2004
Abstract
Previously, spontaneous rifampin resistance mutations were isolated in cluster I of the rpoB gene, resulting in amino acid replacements (Q469R, H482R, H482Y, or S487L) in the Bacillus subtilis RNA polymerase β subunit (W. L. Nicholson and H. Maughan, J. Bacteriol. 184:4936-4940, 2002). In this study, each amino acid change in the β subunit was observed to result in its own unique spectrum of effects on growth and various developmental events, including sporulation, germination, and competence for transformation. The results thus establish the important role played by the RNA polymerase β subunit, not only in the catalytic aspect of transcription, but also in the regulation of major developmental events in B. subtilis.Keywords
This publication has 37 references indexed in Scilit:
- Mapping and sequencing of mutations in the Escherichia colirpoB gene that lead to rifampicin resistancePublished by Elsevier ,2004
- The Spectrum of Spontaneous Rifampin Resistance Mutations in the rpoB Gene of Bacillus subtilis 168 Spores Differs from That of Vegetative Cells and Resembles That of Mycobacterium tuberculosisJournal of Bacteriology, 2002
- Activation of Antibiotic Biosynthesis by Specified Mutations in the rpoB Gene (Encoding the RNA Polymerase β Subunit) of Streptomyces lividansJournal of Bacteriology, 2002
- Detection of Rifampin-Resistant Mycobacterium tuberculosis in Sputa by Nested PCR-Linked Single-Strand Conformation Polymorphism and DNA SequencingJournal of Clinical Microbiology, 2001
- Mutations in the rpoB Gene of Rifampin-Resistant Mycobacterium tuberculosis Isolates in Spain and Their Rapid Detection by PCR–Enzyme-Linked Immunosorbent AssayJournal of Clinical Microbiology, 2001
- The β′ Subunit of Escherichia coli RNA Polymerase Is Not Required for Interaction with Initiating Nucleotide but Is Necessary for Interaction with RifampicinPublished by Elsevier ,2001
- Novel Approach for Improving the Productivity of Antibiotic-Producing Strains by Inducing Combined Resistant MutationsApplied and Environmental Microbiology, 2001
- Genetic and Transcriptional Organization of the Region Encoding the β Subunit of Bacillus subtilis RNA PolymeraseJournal of Biological Chemistry, 1995
- Nucleotide sequence of the Bacillus subtilis trpE and trpD genesGene, 1984
- TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATEProceedings of the National Academy of Sciences, 1958