Fragment Couplings with Stepwise Couplings in Solid-Phase Synthesis. Synthesis of a Fragment of Human Leukocyte Interferon.

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Abstract
A fragment of the human leukocyte interferon was synthesized on a solid-phase. In addition to stepwise couplings, 2 BOC-protected [tert-butyloxycarbonyl] tetrapeptides were added to the growing chain. These peptides, BOC-Phe-Gln-Arg(Tos)-Ile and BOC-Ile-Leu-Ala-Val were prepared on a chloromethyl resin and released by hydrogenolysis. The crude peptide, obtained after HF-cleavage, was purified by gel filtration, partition chromatography and chromatography on LH-20. The ease of purification was no doubt the result of the reduced number of chemical transformations achieved by the use of the fragments. The use of simple peptides, prepared by hydrogenolysis, can be of significant value to increase the quality and yield by peptides synthesized mainly by stepwise methods.