SYNERGISTIC EFFECT OF ALLOANTIBODIES OR F(ab')2 AND PREDNISONE ON MURINE SPLIT HEART ALLOGRAFT SURVIVAL

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Abstract
Heterotopic grafting of split allogeneic mouse hearts across a strong H-2 barrier (A/Sn .fwdarw. A.CA) was used in a study of alloantibody or F(ab'')2 induced enhancement. Strong specific binding of alloantibodies occurred in the graft. A marked synergistic effect on graft survival was observed after combined treatment of recipients with enhancing serum and prednisone. The highly dose-dependent enhancing effect of F(ab'')2 was also strongly potentiated by supporting prednisone treatment. In vitro pre-incubation of the heart tissue with alloantiserum or F(ab'')2 did not prolong graft survival; in contrast, C[complement]-mediated damage of the graft was suggested when serum was used.