Yolk-sac Perfusion of Chick Embryos: Effects of Various Media on Survival

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Abstract
A technique is described which permits the continuous or inter-mittent passage of fluids through the yolk sac of 4-day chick embryos. When a yolk-free, balanced salt solution was perfused for 8 hours at 05 ml/minute, alternating with 8 hours of non-perfusion, almost all embryos died between 20 and 45 hours. Survival time increased in proportion to yolk concentration up to 8% yolk, at which level some embryos were alive 96 hours after perfusion was started.

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