A method for the determination of d-galactosamine in the presence of d-glucosamine
- 1 July 1963
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 88 (1) , 132-136
- https://doi.org/10.1042/bj0880132
Abstract
The determination of D-galactosamine is carried out by condensation of the amino sugar with acetylacetone in anhydrous methanol in the presence of triethylamine and pyridine, transformation of the intermediate formed into 2-methylpyrrole in borate buffer, reaction of the distilled chromogen with p-dimethylaminobenzaldehyde and spectrophotometric measurement of the product at 545 m[mu]. Interference by D-glucosamine is less than 2%. Some compounds formed during the condensation have been isolated. The mechanism of the reaction is discussed.Keywords
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