Pollen Tubes in the Style after Chloral Hydrate and Aceto-Carmine

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Abstract
In styles, which contain a conducting tissue, the pollen tubes can be stained with aceto-carmine and so distinguished from the conducting tissue. Pollinated styles are macerated in a saturated aqueous solution of chloral hydrate. The time of treatment is specific for each kind of style (0.5-24 hr). The stigma, with its conducting tissue, is dissected under a dissecting microscope, placed on a slide, a drop of aceto-carmine added and the preparation covered with a cover slip. By a slight pressure on the cover slip, the conducting tissue is spread out. Aceto-carmine is added from time to time during 24 hr, and the pollen tubes, especially their ends, stain a deep red. The cells of the conducting tissue are only faintly stained. In styles which have a length of only a few millimeters, the pollen tubes can be seen without dissecting the conducting tissue. The preparations can be made relatively permanent by sealing the covers with Krönig'schem Glasskitt (Hollborn und Söhne, Leipzig, Germany).

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