Lysosomal Triglyceride Lipase from the Lateral Line Tissue of Rainbow Trout (Salmo gairdneri)

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Abstract
An acid lipase active toward tripalmitin and having the characteristics of lysosomal enzymes was shown to occur in the red lateral line tissue of rainbow trout (Salmo gairdneri). The enzyme showed maximum activity at pH 4–4.5. Triton X-100 (0.2–2.0%) strongly stimulated the activity of the acid lipase, but it inhibited markedly the lipolytic activity above pH 7. NaF (20 mM) and Na-p-chloromercuriphenyl-sulfonate (1 mM) partially inhibited the acid lipase. Fractionation of the total homogenate by differential centrifugation in 0.25 M sucrose showed that the acid lipase was present at highest concentration in the light mitochondrial fraction. Palmitic acid and dipalmitin were the two main products of hydrolysis of tripalmitin.

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