Salmonella -Induced Filament Formation Is a Dynamic Phenotype Induced by Rapidly Replicating Salmonella enterica Serovar Typhimurium in Epithelial Cells
Open Access
- 1 February 2005
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 73 (2) , 1204-1208
- https://doi.org/10.1128/iai.73.2.1204-1208.2005
Abstract
Salmonella enterica serovar Typhimurium has the fascinating ability to form tubular structures known as Salmonella -induced filaments (Sifs) in host cells. Here, we show that the prevalence of the Sif phenotype in HeLa cells is affected by host cell density, growth, and the multiplicity of infection. Sif formation was observed in cells that displayed rapid intracellular bacterial replication and was found to be dynamic, being maximal 8 to 10 h postinfection and declining thereafter. The virulence factors SpvB and SseJ were found to negatively modulate Sif formation. Our findings demonstrate the complex and dynamic nature of the Sif phenotype.Keywords
This publication has 40 references indexed in Scilit:
- The related effector proteins SopD and SopD2 from Salmonella enterica serovar Typhimurium contribute to virulence during systemic infection of miceMolecular Microbiology, 2004
- Salmonella redirects phagosomal maturationCurrent Opinion in Microbiology, 2004
- Dynamics of bacterial growth and distribution within the liver duringSalmonellainfectionCellular Microbiology, 2003
- Salmonella type III effectors PipB and PipB2 are targeted to detergent‐resistant microdomains on internal host cell membranesMolecular Microbiology, 2003
- Salmonella typhimurium SifA Effector Protein Requires Its Membrane-anchoring C-terminal Hexapeptide for Its Biological FunctionJournal of Biological Chemistry, 2003
- The Salmonella enterica Serovar Typhimurium Translocated Effectors SseJ and SifB Are Targeted to the Salmonella -Containing VacuoleInfection and Immunity, 2003
- SseF and SseG are translocated effectors of the type III secretion system ofSalmonellapathogenicity island 2 that modulate aggregation of endosomal compartmentsCellular Microbiology, 2002
- Disruption of the Salmonella-Containing Vacuole Leads to Increased Replication of Salmonella enterica Serovar Typhimurium in the Cytosol of Epithelial CellsInfection and Immunity, 2002
- The spvB gene‐product of the Salmonella enterica virulence plasmid is a mono(ADP‐ribosyl)transferaseMolecular Microbiology, 2000
- Control of DNA Replication and Cell Proliferation in Eukaryotes by AphidicolinToxicologic Pathology, 1984