Establishment of Two Cell Lines from the Mosquito Toxorhynchites Amboinensis (Diptera: Culicidae) and their Susceptibility to Infection with Arboviruses1

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Abstract
Two cell lines (TA-42 and TA-9) established from Toxorhynchites amboinensis eggs and larvae, respectively, are described. The TA-42 cells are epithelioid in character, while the TA-9 cells are fibroblastic in appearance. Both were initiated and grown in MM/VPI2 medium. Both cell types form a monolayer on glass or plastic. Their origin and identity were confirmed by isozyme analyses. Their susceptibility to infection with 23 different arboviruses was tested. Eleven of the viruses replicated in the TA-42 cells, while 13 grew in the TA-9 cells. Highest postinfection virus titers in both cell lines were obtained with 2 orbiviruses, Changuinola and Corriparta. No cytopathic effect was produced in either cell line by any of the viruses.

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