Application of new catalytic phosphate protecting groups for the highly efficient phosphotriester oligonucleotide synthesis
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (16) , 6525-6540
- https://doi.org/10.1093/nar/14.16.6525
Abstract
An effective procedure for the synthesis of oligonucleotides by the phosphotriester method has been developed. The procedure is based on the use of phosphate protecting groups enabling O-nucleophilic intramolecular catalysis in the reaction of internucleotide bond formation under the action of arylsulfonyl chlorides and their derivatives. Using this new procedure, the time needed to perform one elongation step on polymer support is 7–8 min. The effectiveness of the methodology has been demonstrated in the syntheses of many oligodeoxyribonucleotides of different length with high yields.Keywords
This publication has 5 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Highly efficient oligodeoxyribonucleotide synthesis using fully base protected phosphodiester building blocks carrying 2-(l-methylimidazol-2-yl)phenyl protection of the phosphateNucleic Acids Research, 1986
- Improved rapid phosphotriester synthesis of oligodeoxyribonueleotides using oxygen-nucleoplillic cataiystsNucleic Acids Research, 1985
- Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester methodNucleic Acids Research, 1983
- New effective method for the synthesis of oligonucleotides via phosphotriester intermediatesNucleic Acids Research, 1982