Dimethyl Sulfoxide As a Protective Agent During Freezing and Thawing of Human Spermatozoa.

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Abstract
Summary Dimethyl sulfoxide (DMSO) was evaluated, in terms of motility, as a freeze-thaw protective agent with human spermatozoa. DMSO was found to be equal to glycerol in this protective capacity but proved more toxic during storage at 22°C both before freezing and after thawing. An interaction of protective and toxic effects was noted in a comparison of 5, 10, 15 and 20% DMSO which suggested the 10% level as the most favorable. On the basis of findings, DMSO is not recommended as a substitute for glycerol in the preservation of human spermatozoa by freezing with the methods described. The superior technical assistance of Mr. Kyung Soo Kim is gratefully acknowledged, as is the cooperation of 16 medical students who donated semen.

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