Inhibition of Expression of Natural UAG Suppressor Glutamine tRNA in HIV-Infected Human H9 Cells In Vitro by Avarol
- 1 January 1988
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 4 (4) , 279-286
- https://doi.org/10.1089/aid.1988.4.279
Abstract
HTLV-IIIB-infected H9 cells are shown to contain a high level of the natural UAG suppressor glutamine tRNAGlnUmUg; this tRNA has been demonstrated to be required for the synthesis of Moloney murine leukemia virus (Mo-MuLV)-encoded protease. After cultivation of HTLV-IIIB-infected H9 cells with Avarol at a concentration (1 (μg/ml), previously found to protect the cells against the cytopathic effects of HTLV-III, an almost complete inhibition of the synthesis of the tRNAGlnUmUg was observed. Moreover, we obtained some evidence that the processing of the HTLV-III precursor protein p53 to p24 is inhibited by Avarol in infected cells, suggesting that the compound interferes with the expression of the viral protease gene.Keywords
This publication has 29 references indexed in Scilit:
- Cytochalasin B selectively releases ovalbumin mRNA precursors but not the mature ovalbumin mRNA from hen oviduct nuclear matrixEuropean Journal of Biochemistry, 1987
- Occurrence of Novel Small RNAs with Concomitant Inhibition of Host Cellular U Small Nuclear RNA Synthesis in Vero Cells Infected with Herpes Simplex Virus Type 1Journal of General Virology, 1986
- Trans-activation of human immunodeficiency virus occurs via a bimodal mechanismCell, 1986
- Biphasic and differential effects of the cytostatic agents avarone and avarol on DNA metabolism of human and murine T and B lymphocytesEuropean Journal of Cancer and Clinical Oncology, 1986
- Complete nucleotide sequence of the AIDS virus, HTLV-IIINature, 1985
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970