Effect of freezing on the viability of Mycobacterium paratuberculosis in bovine feces

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Abstract
Three bovine fecal [diagnostic] specimens were cultured for M. paratuberculosis before freezing and after frozen storage at -70.degree. C for 3 and 15 wk. The losses in viability from 0-3 wk of storage were significant (P = 0.01) for all three samples. The losses in viability from 3-15 wk of storage were not significant (P > 0.05) for 2 specimens in which the M. paratuberculosis occurred naturally, but the loss was significant (P = 0.01) in a simulated specimen (M. paratuberculosis culture added to feces from a healthy cow).