16S rRNA sequence analysis of an isolate of Mycobacterium haemophilum from a heart transplant patient

Abstract
Summary Biopsy samples from a heart transplant patient with cellulitis and bursitis yielded an isolate of Mycobacterium haemophilum. The isolate was identified on the basis of a growth requirement for haemin or ferric ammonium citrate, growth at 30°C but not at 37°C, negative catalase test, intracellular growth in McCoy fibroblasts and sequence identity with a portion of the 16S rRNA sequence of the type strain. In comparisons with known 16S rRNA sequences, M. haemophilum grouped with other pathogenic, slow-growing mycobacteria, showing close sequence similarity to M. marinum (98·8%) and lower similarity to M. ulcerans and M. tuberculosis complex organisms. M. haemophilum and M. marinum share other features including optimal growth at 30°C and the ability to cause superficial skin lesions in man.

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