Metal Cation/Anion Speciation via Paired-Ion, Reversed Phase HPLC with Refractive Index and/or Inductively Coupled Plasma Emission Spectroscopic Detection Methods

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Abstract
Conventional high performance liquid chromatography [HPLC] instrumentation and packing materials can be inexpensively and rapidly utilized for the qualitative and quantitative analysis of various toxic metal cations or anions. The final approaches utilize reversed phase HPLC in the form of paired-ion separations. The detection of individually eluted, fully resolved metal cations or anions is possible via conventional refractive index or inductively coupled plasma emission spectroscopic detection. In many cases, unresolved mixtures of metal cations, eluted as a single peak on HPLC, can be resolved and identified via the use of ICP [inductively coupled plasma] detection. Both metal cations and anions can be easily resolved, according to oxidation states, using paired-ion techniques, in combination with ICP detection. Final data representation can be in the form of conventional, continuous RI [refractive index] and/or ICP chromatograms, via pulsed data ICP presentations and/or via tabular ICP data presentation.