Numerical Taxonomy of Pseudomonas alcaligenes, P. pseudoalcaligenes, P. mendocina, P. stutzeri, and Related Bacteria

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Abstract
A numerical phenotypic analysis, in which the unweighted pair group average linkage method and Dice similarity coefficient were used, was performed on 155 strains received as Pseudomonas alcaligenes, Pseudomonas pseudoalcaligenes, Pseudomonas mendocina, or Pseudomonas stutzeri. These organisms are the clinically important nonfluorescent species belonging to ribosomal ribonucleic acid group I of Palleroni and co-workers. Six major clusters, which could be further divided into 20 subclusers, were formed. Most strains received as P. alcaligenes fell into three subclusters (subclusters A1, A2, and B1), whereas strains received as P. pseudoalcaligenes were mainly classified in two other subclusters (subclusters C2 and C3). All but two strains (subcluster D1) of organisms received as P. mendocina were grouped in subcluster D2. Most of the 45 strains received as P. stutzeri were contained in a large subcluster, subcluster E2 (39 strains). strains belonging to fluorescent pseudomonad species (Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas putida), which were included in the analysis for control purposes, were contained in one cluster, which comprised seven subclusters.