Intracellular transport of microinjected 5S and small nuclear RNAs
- 1 February 1982
- journal article
- Published by Springer Nature in Nature
- Vol. 295 (5850) , 572-577
- https://doi.org/10.1038/295572a0
Abstract
The mechanism by which some RNAs are segregated in the cell nucleus was analysed by microinjecting 32 P-labelled total RNA from HeLa cells into the cytoplasm of Xenopus oocytes. Small nuclear RNAs (u1, U2, U4, U5 and U6) migrated into the cell nucleus, where they became 30-60 fold more concentrated than in the cytoplasm. Other RNAs, such as tRNA and 7S RNA, remained in the cytoplasm, while 5S RNA became concentrated in the nucleolus. Studies with lupus erythematosus antibodies showed that the migrating RNAs become associated with oocyte RNA-binding proteins.Keywords
This publication has 33 references indexed in Scilit:
- Two groups of small stable RNAsCell, 1981
- RNA processing in microinjected Xenopus oocytesJournal of Molecular Biology, 1981
- Order and intracellular location of the events involved in the maturation of a spliced tRNANature, 1980
- Transcription of cloned tRNA genes and the nuclear partitioning of a tRNA precursorCell, 1979
- Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytesNature, 1979
- The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cellsCell, 1978
- Intracellular migration of nuclear proteins in Xenopus oocytesNature, 1978
- Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.The Journal of cell biology, 1975
- Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.The Journal of cell biology, 1975
- Low-Molecular-Weight Nuclear RNA'sPublished by Elsevier ,1974