T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries
- 1 August 2006
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 6 (15) , 4210-4222
- https://doi.org/10.1002/pmic.200500606
Abstract
We investigated whether the T7 system of phage display could produce peptide libraries of greater diversity than the M13 system of phage display due to the differing processes of lytic and filamentous phage morphogenesis. Using a bioinformatics‐assisted computational approach, collections of random peptide sequences obtained from a T7 12‐mer library (X12) and a T7 7‐mer disulfide‐constrained library (CX7C) were analyzed and compared with peptide populations obtained from New England BioLabs' M13 Ph.D.‐12™ and Ph.D.‐C7C™ libraries. Based on this analysis, peptide libraries constructed with the T7 system have fewer amino acid biases, increased peptide diversity, and more normal distributions of peptide net charge and hydropathy than the M13 libraries. The greater diversity of T7‐displayed libraries provides a potential resource of novel binding peptides for new as well as previously studied molecular targets. To demonstrate their utility, several of the T7‐displayed peptide libraries were screened for streptavidin‐ and neutravidin‐binding phage. Novel binding motifs were identified for each protein.Keywords
This publication has 23 references indexed in Scilit:
- Rapid isolation of high-affinity protein binding peptides using bacterial displayProtein Engineering, Design and Selection, 2004
- RELIC – A bioinformatics server for combinatorial peptide analysis and identification of protein‐ligand interaction sitesProteomics, 2004
- The use of mRNA display to select high-affinity protein-binding peptidesProceedings of the National Academy of Sciences, 2001
- Screening of cyclic peptide phage libraries identifies ligands that bind streptavidin with high affinitiesBiochemistry, 1995
- Identification of avidin and streptavidin binding motifs among peptides selected from a synthetic peptide library consisting solely of D‐amino acidsJournal of Peptide Science, 1995
- Antibody as a surrogate receptor in the screening of a phage display libraryGene, 1993
- An M13 phage library displaying random 38-amino-acid peptides as a source of novel sequences with affinity to selected targetsGene, 1993
- M13 bacteriophage displaying disulfide-constrained microproteinsGene, 1993
- A new type of synthetic peptide library for identifying ligand-binding activityNature, 1991
- Random Peptide Libraries: a Source of Specific Protein Binding MoleculesScience, 1990