In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2 H - Tetrazolium Hydroxide (XTT) Assay
- 1 July 2004
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 48 (7) , 2742-6
- https://doi.org/10.1128/aac.48.7.2742-2746.2004
Abstract
Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of the isolates tested are inhibited [MIC(50)]) of 0.06 to 0.13 mg/liter) was superior to amphotericin B (MIC(50) of 0.5 to 1.0 mg/liter).Keywords
This publication has 25 references indexed in Scilit:
- Molecular Detection and Identification of Agents of Eumycetoma: Detailed Report of Two CasesJournal of Clinical Microbiology, 2003
- Madurella mycetomatisStrains from Mycetoma Lesions in Sudanese Patients Are ClonalJournal of Clinical Microbiology, 2003
- Mycetoma of the Foot Caused byFusarium solani: Identification of the Etiologic Agent by DNA SequencingJournal of Clinical Microbiology, 2003
- Comparison of Spectrophotometric and Visual Readings of NCCLS Method and Evaluation of a Colorimetric Method Based on Reduction of a Soluble Tetrazolium Salt, 2,3-Bis {2-Methoxy-4-Nitro-5-[(Sulfenylamino) Carbonyl]-2H- Tetrazolium-Hydroxide}, for Antifungal Susceptibility Testing of Aspergillus SpeciesJournal of Clinical Microbiology, 2001
- In Vitro Activities of 10 Antifungal Drugs against 508 Dermatophyte StrainsAntimicrobial Agents and Chemotherapy, 2001
- Utility of 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenyl-Amino)Carbonyl]-2 H -Tetrazolium Hydroxide (XTT) and Minimum Effective Concentration Assays in the Determination of Antifungal Susceptibility of Aspergillus fumigatus to the Lipopeptide Class of CompoundsJournal of Clinical Microbiology, 2001
- Improvement of eumycetoma with itraconazoleJournal of the American Academy of Dermatology, 1997
- TREATMENT OF EUMYCETOMA WITH KETOCONAZOLEAustralasian Journal of Dermatology, 1993
- Tropical MycosesChemotherapy, 1992
- Treatment of Paracoccidioidomycosis, Candidiasis, Chromomycosis, Lobomycosis, and Mycetoma with KetoconazoleInternational Journal of Dermatology, 1980