HEMATOPOIETIC STEM-CELL PROLIFERATIVE BEHAVIOR AS REVEALED BY BROMODEOXYURIDINE LABELING

  • 1 January 1980
    • journal article
    • research article
    • Vol. 8  (8) , 1075-1079
Abstract
Hematopoietic stem cells (CFUs) are thought to represent a heterogeneous population with different probabilities of self-renewal and different rates of proliferation. As an approach to further characterization of this population, we determined the disappearance of bromodeoxyuridine (BrdUrd)-induced CFUS sensitization to UV light in normal mice infused with BrdUrd for 3 wk and in hydroxyurea-pretreated mice regenerating their CFUS pool during a 1 wk BrdUrd infusion. The same exponential disappearance with a T1/2 of 6 days was found in each case. From this and from the apparent absence of a secondary slope on the sensitization decay curve for the recovered hydroxyurea-treated bone marrow, it was concluded that fewer than 10% of CFUS may be mitotically quiescent for prolonged periods and that the age structure of the CFUS population reflects a relatively short proliferative history.

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