Isolation and Characterization of Subspecies of Murine Interferon Alpha

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Abstract
Interferon produced by mouse L-929 cells by incubation with poly(I.cntdot.c) is known to be composed of a mixture of MuIFN-.alpha. [murine interferon-.alpha.] and MuIFN-.beta.. The .alpha. component was separated from the .beta. species by affinity chromatography over a monoclonal anti-MuIFN-.beta. agarose column and partially purified by gel filtration. MuIFN-.alpha., prepared by this method was separated into at least 5 ssp. by chromatofocusing. The approximate pI [isoelectric point] values of these components are .gtoreq. 7.5, 6.5, 6.2, 5.9 and 5.6, respectively. Component 3 (pI 6.2) was the most prominent subspecies present in the MuIFN-.alpha. preparations, representing 40-50% of the total antiviral activity. Component 1 (pI .gtoreq. 7.5) which accounted for .apprx. 5% of the antiviral activity on mouse cells, differed in some properties from the other interferon subspecies. It showed a relatively high antiviral activity on heterologous cells and it was eluted from a Sephadex column after the other .alpha. subspecies. It showed a diminished binding to heparin as compared to the other MuIFN-.alpha. subspecies, indicating a lower affinity for polynucleotides.

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