Active sites of the cyclic GMP phosphodiesterase γ‐subunit of retinal rod outer segments
- 18 July 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 234 (2) , 287-290
- https://doi.org/10.1016/0014-5793(88)80100-5
Abstract
Monoclonal antibodies were prepared to the γ-subunit of the cGMP phosphodiesterase. One of them γp-1, suppresses the activation of phosphodiesterase through the α-subunit of transducin. The γ-subunit fragment 24–45 rich in Arg and Lys residues is involved in γp-1 binding and is essential for the γ-subunit interaction with transducin. Carboxypeptidase Y cleaves off seven amino acid residues from the C-terminus of the γ-subunit resulting in phosphodiesterase activation. Thus, the C-terminal fragment of γ-subunit participates in phosphodiesterase inhibition.Keywords
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