CD98 induces LFA‐1‐mediated cell adhesion in lymphoid cells via activation of Rap1
Open Access
- 31 January 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 489 (2-3) , 249-253
- https://doi.org/10.1016/s0014-5793(00)02222-5
Abstract
CD98 is a multifunctional heterodimeric membrane protein involved in the regulation of cell adhesion as well as amino acid transport. We show that CD98 cross‐linking persistently activates Rap1 GTPase in a LFA‐1‐dependent manner and induces LFA‐1/ICAM‐1‐mediated cell adhesion in lymphocytes. Specific phosphatidylinositol‐3‐kinase (PI3K) inhibitors suppressed both LFA‐1 activation and Rap1GTP generation, and abrogation of Rap1GTP by retroviral over‐expression of a specific Rap1 GTPase activating protein, SPA‐1, totally inhibited the LFA‐1/ICAM‐1‐mediated cell adhesion. These results suggest that CD98 cross‐linking activates LFA‐1 via the PI3K signaling pathway and induces accumulation of Rap1GTP in a LFA‐1‐dependent manner, which in turn mediates the cytoskeleton‐dependent cell adhesion process.Keywords
This publication has 25 references indexed in Scilit:
- Novel molecular mechanisms of dendritic cell-induced T cell activationInternational Immunology, 2000
- The immunological synapse and the actin cytoskeleton: molecular hardware for T cell signalingNature Immunology, 2000
- Amino acid transport of y+L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid transporter familyThe EMBO Journal, 1999
- All in the family? New insights and questions regarding interconnectivity of Ras, Rap1 and RalThe EMBO Journal, 1998
- Identification and Characterization of a Membrane Protein (y+L Amino Acid Transporter-1) That Associates with 4F2hc to Encode the Amino Acid Transport Activity y+LPublished by Elsevier ,1998
- Human SPA-1 Gene Product Selectively Expressed in Lymphoid Tissues Is a Specific GTPase-activating Protein for Rap1 and Rap2Journal of Biological Chemistry, 1997
- Galectin‐3 stimulates uptake of extracellular Ca2+ in human Jurkat T‐cellsFEBS Letters, 1996
- Role of Rho in Chemoattractant-Activated Leukocyte Adhesion Through IntegrinsScience, 1996
- Ligand intercellular adhesion molecule 1 has a necessary role in activation of integrin lymphocyte function-associated molecule 1.Proceedings of the National Academy of Sciences, 1993
- Regulatory mechanisms in leukocyte adhesion: flexible receptors for sophisticated travelersImmunology Today, 1992