Cell solute composition and potassium effects in slices of the rectal gland of the dogfish shark (Squalus acanthias)

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Abstract
Cell solute composition and apparent intracellular ionic concentrations (subscript i) were determined in rectal gland slices of the dogfish shark (Squalus acanthias). 1. On aerobic incubation in elasmobranch Ringer's at 15 °C, the steady state values were: K i + , 151 mM, close to its apparent electrochemical equilibrium; Ca i 2+ , 1.7 mM; Mg i 2+ , 7.5 mM; Cl i − , 97 mM (maintained against its electrochemical potential gradient); the sum (Na i + +K i + ) was about 66 meq/kg cell water lower than in the medium. Of free amino acids, only the effectively impermeable anionic taurine (32 mM) and glutamate (3.7 mM) significantly contribute to the Donnan system. 2. Variations of the Ringer's K+ concentrations, or the presence of 0.5 mM ouabain, demonstrated the permeability of the cell membrane to Na+, K+, and Cl−. 3. Cell volume changes: 0.5 mM ouabain produced a gradual swelling and ionic changes consistent with an inhibition of the sodium pump. In high-K+ media the cells swelled massively and the membrane depolarized. A linear relationship between cell volume changes and the Nernst potential for K+ was found. These properties are consistent with a pump-and-leak system for cell volume maintenance. 4. The K+-induced cell swelling was found to be reversible on transfer of the tissue to regular Ringer's. The extrusion of cell water was associated with an influx of Na+ into, and an efflux of K+ from, the cells (both fluxes down-hill), as well as an extrusion of Cl− against its concentration gradient. Observed changes of the Nernst potentials for K+ and Cl− indicate that the reversibility of the K+-induced swelling is accompanied by cell membrane repolarization. These observations suggest the involvement of an additional mechanism for cell volume maintenance.

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