Successful Culture in vitro of Bovine Embryos to the Blastocyst Stage
Open Access
- 1 March 1976
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 14 (2) , 157-162
- https://doi.org/10.1095/biolreprod14.2.157
Abstract
Two hundred and sixty bovine embryos between the two- and eight-cell stage were collected from synchronized, PMSG-treated cows. Embryos were placed randomly in culture among 11 media and 2 gas atmospheres and development was recorded at 24 h intervals for the duration of culture. The number of cells present at the cessation of culture was determined following aceto-orcein staining.Keywords
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