Demethylated CD8 Gene in CD4 + T Cells Suggests that CD4 + Cells Develop from CD8 + Precursors
- 25 November 1988
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 242 (4882) , 1174-1176
- https://doi.org/10.1126/science.2460926
Abstract
Mature T cells and medullary thymocytes bear either the CD4 or CD8 differentiation antigen. Precursor cells in the thymus express neither CD4 nor CD8 (CD4-8-), but most cortical thymocytes are CD4+8+. Whether CD4+ and CD8+ mature T cells arise directly from CD4-8- precursors or from a CD4+8+ intermediate remains unresolved. In this study, methylation of the CD8 gene in murine T cells and thymocytes was examined. There was progressive demethylation of the CD8 gene in the thymus during the transition from CD4-8- to CD4+8+. A similar pattern of demethylation of the CD8 gene was seen in CD4+ mature T cells, suggesting previous expression of CD8 in the CD4+ lineage.This publication has 15 references indexed in Scilit:
- Tolerance in T-cell-receptor transgenic mice involves deletion of nonmature CD4+8+ thymocytesNature, 1988
- CD4+ murine T cells develop from CD8+ precursors in vivoNature, 1987
- Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily.The Journal of Experimental Medicine, 1987
- Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNACell, 1985
- Early T lymphocytes. Differentiation in vivo of adult intrathymic precursor cells.The Journal of Experimental Medicine, 1985
- Cell Surface Antigen Expression on Thymocytes: Development and Phenotypic Differentiation of Intrathymic SubsetsImmunological Reviews, 1984
- T Cell Development in the Adult Murine Thymus: Changes in the Expression of the Surface Antigens Ly2, L3T4 and B2A2 during Development from Early Precursor Cells to EmigrantsImmunological Reviews, 1984
- The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. An antibody to a receptor allotype.The Journal of Experimental Medicine, 1984
- Precursors of T cell growth factor producing cells in the thymus: ontogeny, frequency, and quantitative recovery in a subpopulation of phenotypically mature thymocytes defined by monoclonal antibody GK-1.5.The Journal of Experimental Medicine, 1983
- Cloned T Lymphocytes and Monoclonal Antibodies as Probes for Cell Surface Molecules Active in T Cell‐Mediated CytolysisImmunological Reviews, 1982