In vitro Incorporation of Iron-59 into Hemoglobin S Visualized by Autoradiography.

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Abstract
Human bone marrow of a patient with sickle cell anemia and peripheral blood of a normal individual were incubated with Fe59 under similar conditions. Autoradiograms were prepared from the cells. After incubation, hemolysates of the cells were fractionated by paper electrophoresis and the radioactivity of the fractions was measured. Ninety per cent of total radioactivity was located in hemoglobin S from bone marrow hemolysates compared to less than 5% in hemoglobin A from normal peripheral blood. The rate of hemoglobin synthesis as measured by Fe59 utilization was more rapid in the most immature erythrocytic cells begin[long dash]ning with the rubriblast and occurred in or adjacent to the nucleus. Rate of synthesis at 0[degree] C was 25% of that at 37[degree] C.

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