A Cytotoxic Antiglobulin Technique for Assay of Antibodies to Histocompatibility Antigens

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Abstract
Summary: A method for increasing the cytotoxic activity of antibodies to lymphoid cells has been developed. Mouse isoantisera with 7 S and 18 S antibody activity were tested against an allogeneic lymphoid tumor cell. A variety of heterologous antiglobulins with different specificites were examined for their ability to produce inceased cytotoxicity. Both anti-IgG and anti-light chain reagents increased the cytotoxic titers of the 7 S antibody as much as 16-fold. Antibodies against mouse light chains were also able to enhance the cytotoxicity of the 18 S antibody. The degree of enhancement of cytotoxicity was considerably less than that found for antiglobulin reactions with erythrocytes and the possible explanations for these differences were discussed.

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