Transcription of insertion elements IS1 and IS2 in vitro

Publisher Website
Google Scholar
Abstract
Insertion elements IS1 and IS2 integrated within thegal operator-promoter region, an IS1 element in genegalT and insertions IS1 and IS2 integrated in thexycIIOP region of phage λ were transcribed in vitro withE. coli RNA-polymerase. The insertion elements are transcribed exclusively by polymerase molecules started at thegal promoter and the λPR promoter respectively. No promoter exists on IS1 or IS2 which can be recognized by RNA-polymerase in the pure in vitro transcription system used. Both insertions apparently are transcribed with a lower elongation rate thangal operon DNA or λDNA. RNAs transcribed from the termini of IS1 and IS2 respectively were analysed by hybridization experiments. They are different in sequence.