SIMPLIFICATION OF THE ROE AND KUETHER PROCEDURE FOR ASCORBIC ACID DETERMINATION IN ADRENAL GLANDS
- 1 November 1951
- journal article
- research article
- Published by Bioscientifica in Acta Endocrinologica
- Vol. 8 (3) , 247-250
- https://doi.org/10.1530/acta.0.0080247
Abstract
By increasing the temp. at which the 2,4-dinitrophenylhydrazine-ascorbic acid coupling takes place from 37[degree]C to 57.4[degree]C the incubation can be reduced from 3 to 3/4 hour; or at 100[degree]C to 3.5 min. At high temp. the time and temp. must be very carefully controlled to obtain uniform results.Keywords
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