The Drosophila gene 2A5 complements the defect in mitochondrial F1‐ATPase assembly in yeast lacking the molecular chaperone Atp11p

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Abstract
Assembly of mitochondrial F1‐ATPase in Saccharomyces cerevisiae requires the molecular chaperone, Atp11p. Database searches have identified protein sequences from Schizosaccharomyces pombe and two species of Drosophila that are homologous to S. cerevisiae Atp11p. A cDNA encoding the putative Atp11p from Drosophila yakuba was shown to complement the respiratory deficient phenotype of yeast harboring an atp11::HIS3 disruption allele. Furthermore, the product of this Drosophila gene was shown to interact with the S. cerevisiae F1 β subunit in the yeast two‐hybrid assay. These results indicate that Atp11p function is conserved in higher eukaryotes.