An improved technique for immunofixation of electrophoretograms.
Open Access
- 1 September 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (9) , 1639-1641
- https://doi.org/10.1093/clinchem/24.9.1639
Abstract
Immunofixation coupled with electrophoresis is winning favor as an easy, sensitive, and versatile method for detecting specific protein fractions. Here, we discuss problems which appear when antisera are applied onto the electrophoretic medium (Cellogel or cellulose acetate). Longitudinal slits can be made in the electrophoretic medium without worsening resolution. Such longitudinally slitted electrophoretic membranes can be used in electrophoresis and specific antisera applied simply by pipetting them onto the electrophoretograms. The method has been used successfully in typing immunoglobulins.This publication has 1 reference indexed in Scilit: