Characterization of adhesion zones in E. coli cells
- 15 January 1990
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 67 (1-2) , 39-43
- https://doi.org/10.1016/0378-1097(90)90164-l
Abstract
After plasmolysis of Escherichia coli cells, the adhesion zones were characterized using the cytochemical PTA and SP procedures which stain peptidoglycan and lipopolysaccharides (LPS) respectively. A PTA-stained layer was detected at the adhesion sites. This layer was visualized irrespective of the electron microscopy procedure used. Also, using SP staining an outer membrane in which LPS molecules were asymmetrically distributed, was observed.Keywords
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