Production of Antiserum to the Reverse Transcriptase of Mason-Pfizer Monkey Virus

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Abstract
The reverse transcriptase of Mason-Pfizer moneky virus (M-PMV) was isolated and partially purified by ion exchange chromatography. Sera from rabbits immunized with the partially purified enzyme were shown by microimmunodiffusion analysis to be immunologically specific for the M-PMV polymerase. The immune serum also specifically inhibited M-PMV polymerase activity and this inhibitory activity resided in the IgG [immunoglobulin G] fraction of the serum. The application of these reagents to examining virus identity and investigating the possible viral etiology of human breast cancer is discussed.

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