HORMONE BINDING BY HUMAN MAMMARY-CARCINOMA

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (7) , 1946-1952
Abstract
The specific binding of labeled porcine insulin, human prolactin and human growth hormone was studied in 63 human breast tumors and 15 nonmalignant breast tissues. Most (90%) of the tumors demonstrated significant binding of insulin, as did 80% of nonmalignant tissues. Autoradiographic studies indicated that insulin bound dominantly to tumor cells, rather than to fat and fibrous tissue contained within tumors. Specific binding of prolactin and growth hormone of > 1% was seen in 20 and 12% of tumors, respectively, and 1 tumor studied in detail showed a small amount of saturable, high affinity prolactin binding. The affinity of binding of insulin and prolactin to tumor was similar to that seen in target tissues in subprimate species (Kd = 4 .times. 10-10 M), but the prolactin binding capacity in the 1 tumor studied in detail was very low (10 fmol [femto moles]/mg membrane protein), compared with prolactin responsive experimental mammary carcinoma.

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