Cell isolation procedures matter: a comparison of different isolation protocols of bone marrow mononuclear cells used for cell therapy in patients with acute myocardial infarction
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- 28 February 2007
- journal article
- research article
- Published by Oxford University Press (OUP) in European Heart Journal
- Vol. 28 (6) , 766-772
- https://doi.org/10.1093/eurheartj/ehl509
Abstract
Aim The recently published REPAIR-AMI and ASTAMI trial showed differences in contractile recovery of left ventricular function after infusion of bone marrow-derived cells in acute myocardial infarction. Since the trials used different protocols for cell isolation and storage (REPAIR-AMI: Ficoll, storage in X-vivo 10 medium plus serum; ASTAMI: Lymphoprep, storage in NaCl plus plasma), we compared the functional activity of BMC isolated by the two different protocols.Keywords
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