Structural analysis of mitochondrial pores
- 1 February 1990
- journal article
- review article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 46 (2) , 137-145
- https://doi.org/10.1007/bf02027309
Abstract
Structural information about the channel in the mitochondrial outer membrane, derived from sequence analysis and electron microscopy of two-dimensional crystals, is summarized. A model for the channel is presented, consisting of a cylindrical beta-barrel that is formed by one or two 30-kDa polypeptides, with an alpha-carbon backbone diameter of 3.8 nm. The radial distributions of basic amino acids and lipid-contact regions on the projected cylinder are mapped relative to interchannel bonding sites inferred from channel packing in the arrays. Speculation on the kinds of conformational changes that the channel might undergo is also presented.Keywords
This publication has 49 references indexed in Scilit:
- Diameter of the mitochondrial outer membrane channel: Evidence from electron microscopy of frozen‐hydrated membrane crystalsFEBS Letters, 1989
- Lateral segregation of sterol and channel proteins in the mitochondrial outer membrane induced by phospholipase A2: Evidence from negative-stain electron microscopy using filipinJournal of Ultrastructure and Molecular Structure Research, 1988
- Patch clamping the outer mitochondrial membraneThe Journal of Membrane Biology, 1987
- Effect of succinylation on images of negatively stained arrays of mitochondrial outer membrane channelsJournal of Ultrastructure and Molecular Structure Research, 1986
- Voltage dependence and ion selectivity of the mitochondrial channel, VDAC, are modified by succinic anhydrideThe Journal of Membrane Biology, 1985
- Gap junction structuresJournal of Molecular Biology, 1984
- Pore protein and the hexokinase‐binding protein from the outer membrane of rat liver mitochondria are identicalFEBS Letters, 1982
- A study of positive staining for electron microscopy using collagen as a model system—I. Staining by phosphotungstate and tungstate ionsMicron, 1982
- Reconstitution in planar lipid bilayers of a voltage-dependent anion-selective channel obtained from paramecium mitochondriaThe Journal of Membrane Biology, 1976
- On the impermeability of the outer mitochondrial membrane to cytochrome c. I. Studies on whole mitochondriaBiochimica et Biophysica Acta (BBA) - Biomembranes, 1969