Endothelin-3 Directly Affects Neurons in the Anteroventral Third Ventricle Region and Supraoptic Nucleus of the Rat Hypothalamus in vitro
- 1 January 1991
- journal article
- opening lecture
- Published by Wolters Kluwer Health in Journal of Cardiovascular Pharmacology
- Vol. 17, S200-202
- https://doi.org/10.1097/00005344-199100177-00057
Abstract
Summary Extracellular recordings were made from anteroventral third ventricle (AV3V) and supraoptic nucleus (SON) neurons of rat hypothalamus in slice preparations. ET-3 was applied at concentrations of 10 10 to 3 x 10 7M. Of 119 AV3V neurons tested. 21 (18%) were excited. 8 (6%) were slightly inhibited, and 90 (76%) were unaffected. The threshold concentration to evoke responses was approximately 10-8M. Excitatory responses of the AV3V neurons to ET-3 remained in aCa2+ -free medium. Of 120 SON neurons tested. 46 were phasic (putative vasopressin neuron) and 74 were nonphasic (putative oxytocin neuron). Of 46 phasic neurons tested. 26 (56.5%) were inhibited by ET-3, 20 (43.5%) were nonresponsive. and none was excited. Of 74 nonphasic neurons tested. 14 (19%) neurons were inhibited by ET-3. 60 (81%) were nonresponsive. and none was excited. These results suggest that ET-3 has dual effects on AV3V and SON neurons and the mechanisms of the responses may be different in the AV3V and SON neurons.Keywords
This publication has 0 references indexed in Scilit: