Formation of leukotriene B4 and monohydroxy acids in slices of porcine thyroid gland
- 1 April 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 117 (4) , 463-469
- https://doi.org/10.1530/acta.0.1170463
Abstract
Slices of porcine thyroid gland were incubated with arachidonic acid and ionophore A 23187. This led to the formation of 5-hydroxy-eicosatetraenoic acid (5-HETE), 12-HETE, 15-HETE and leukotriene B4 (LTB4). Time course studies demonstrated that levels of detected LTB4 reached a plateau after 30 min and that in parallel with the synthesis of this compound, greater amounts of 5-HETE was formed. The present results demonstrate the formation of lipoxygenase products in the porcine thyroid gland, indicating possible physiological/pathophysiological roles for these compounds in this organ.This publication has 7 references indexed in Scilit:
- Arachidonate 5-lipoxygenase of porcine leukocytes studied by enzyme immunoassay using monoclonal antibodies.Journal of Biological Chemistry, 1987
- Single protein from human leukocytes possesses 5-lipoxygenase and leukotriene A4 synthase activities.Proceedings of the National Academy of Sciences, 1986
- Arachidonate metabolism in the mouse thyroid implication of the lipoxygenase pathway in thyrotropin actionProstaglandins, 1981
- Novel transformations of arachidonic acid by the rat thyroid in vitroBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- In vitro effects of arachidonic acid and of inhibitors of its metabolism on the dog thyroid glandProstaglandins, 1980
- Arachidonic acid metabolism in polymorphonuclear leukocytesProceedings of the National Academy of Sciences, 1979
- Transformation of arachidonic acid and homo-gamma-linolenic acid by rabbit polymorphonuclear leukocytes. Monohydroxy acids from novel lipoxygenasesJournal of Biological Chemistry, 1976