A systematic study of HLA class II-beta DNA restriction fragments in insulin-dependent diabetes mellitus.
- 1 May 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (10) , 3335-3339
- https://doi.org/10.1073/pnas.82.10.3335
Abstract
DNA restriction fragments of the genes encoding HLA class II-beta antigens were compared in 34 patients with insulin-dependent diabetes mellitus and 34 HLA-DR-matched healthy individuals. Ninety-three fragments, determined by six restriction enzymes (EcoRI, EcoRV, HindIII, BamHI, Pvu II, and Taq I), were analyzed: (i) A DR Taq I 12.7-kilobase-pair fragment might be a marker for the extended haplotype HLA-B8, DR3. (ii) In controls, DR4 haplotypes are associated with two distinct clusters of DQ restriction fragments (DQR4 and DQR5). Almost all (94%) DR4 patients belong to the DQR4 and not to the DQR5 cluster. This suggests that, among HLA-DR4 haplotypes, only DQR4 haplotypes are involved in susceptibility to insulin-dependent diabetes mellitus. (iii) A DR Taq I 14.5-kilobase-pair fragment was found to be strongly associated with DQR4, mainly in DR3/DR4 heterozygous patients (P = 5 X 10(-4). However, these results must be interpreted with caution, taking into account the high number of statistical tests performed.This publication has 14 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Clusters of HLA class II beta restriction fragments describe allelic series.Proceedings of the National Academy of Sciences, 1984
- Extended major histocompatibility complex haplotypes in type I diabetes mellitus.Journal of Clinical Investigation, 1984
- Class II HLA-DC beta-chain DNA restriction fragments differentiate among HLA-DR2 individuals in insulin-dependent diabetes and multiple sclerosis.Proceedings of the National Academy of Sciences, 1984
- Analysis of HLA class I genes with restriction endonuclease fragments: implications for polymorphism of the human major histocompatibility complex.Proceedings of the National Academy of Sciences, 1983
- Genetic mapping of a human class II antigen beta-chain cDNA clone to the SB region of the HLA complex.Proceedings of the National Academy of Sciences, 1983
- HLA-D region β-chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individualsNature, 1983
- Sequences related to HLA-DR alpha chain on human chromosome 6: restriction enzyme polymorphism detected with DC alpha chain probes.Proceedings of the National Academy of Sciences, 1983
- Allelic polymorphism and complexity of the genes for HLA-DR β-chains—direct analysis by DNA–DNA hybridizationNature, 1982
- [15] Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paperPublished by Elsevier ,1979