Cloning of cybB, the gene for cytochrome b561 of Escherichia coli K12
- 1 December 1984
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 198 (1) , 1-6
- https://doi.org/10.1007/bf00328692
Abstract
A 37 kb fragment of DNA from an F-prime factor, F100-12, which showed a gene dosage effect on b-type cytochromes, was cloned with a cosmid vector, pHC79. Gel filtration of cytochromes and product analysis of the hybrid plasmids indicated that this fragment contained cybB, the structural gene for cytochrome b561. A chromosomal DNA fragment carrying the cybB gene was cloned by the plaque hybridization technique with Charon 4A as a vector. The gene was subcloned into pBR322 and was located in a 1.3 kb DNA fragment. It was concluded that the cybB gene is located on the chromosome of Escherichia coli K12.Keywords
This publication has 34 references indexed in Scilit:
- Chromosomal location of the Escherichia coli cytochrome b556 gene, cybAMolecular Genetics and Genomics, 1984
- A small cosmid for efficient cloning of large DNA fragmentsGene, 1980
- A rapid alkaline extraction procedure for screening recombinant plasmid DNANucleic Acids Research, 1979
- Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda.Proceedings of the National Academy of Sciences, 1979
- Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proceedings of the National Academy of Sciences, 1979
- Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA: Preparation and Screening of Shotgun CollectionsScience, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Apparent cytochrome gene dose effects in F-lac and F-gal heterogenotes of Escherichia coliArchives of Biochemistry and Biophysics, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970