The influence of respiratory bacteria and their biochemical fractions onAspergillus fumigatus

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Abstract
Living cells of Pseudomonas aeruginosa, Haemophilus influenzae and Staphylococcus aureus, inhibited hyphal growth in Aspergillus fumigatus. Induced haemolytic activity by Haemophilus and Pseudomonas was observed near fungal colonies, and may signify that a similar mechanism might operate in vivo. The inhibitor was shown to diffuse through cellophane and to retain activity in the absence of living bacterial cells. The inhibitory activity was located in the extracellular slime in the case of Pseudomones and Haemophilus. Extracts of extracellular enzymes, whole cell walls, supernatants from ultrasonicated cells and freeze-thaw whole fractions were not inhibitory for hyphal growth. Inhibition of spore germination was observed in slime fractions and supernatants from ultrasonicated cells in all 3 bacteria. Freeze-thaw, pyocyanine and EDTA slime fractions from Pseudomonas were also inhibitory. Loss of inhibitory activity accompanied methods of fractionation which eliminated protein components.