Restriction endonuclease cleavage map of pKM101: Relationship to parental plasmid R46
- 1 July 1981
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 182 (2) , 268-272
- https://doi.org/10.1007/bf00269669
Abstract
A detailed restriction endonuclease cleavage map of the plasmid pKM101 has been constructed. pKM101 plasmids containing individual Tn5 insertions were used to facilitate the ordering of restriction fragments generated by enzymes cleaving pKM101 at multiple sites. By restriction enzyme analysis, pKM101 (35.4 kilobases) appears to have arisen from its clinically-isolated parent by deletion of a single DNA region which codes for three of the four drug resistances carried by R46.Keywords
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