Pseudomonas aeruginosa ExoU, a Toxin Transported by the Type III Secretion System, Kills Saccharomyces cerevisiae
Open Access
- 1 July 2003
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 71 (7) , 4144-4150
- https://doi.org/10.1128/iai.71.7.4144-4150.2003
Abstract
ExoU, a protein transported by the type III secretion system of Pseudomonas aeruginosa , is an important cytotoxin, though its mechanism of action is unclear. Here we show that the intracellular expression of ExoU is cytotoxic to Saccharomyces cerevisiae . Furthermore, internal amino- and carboxyl-terminal deletions confirmed that regions of ExoU previously shown to be essential for killing mammalian cells were also required for killing yeast cells. These findings indicate that S. cerevisiae is a useful model organism for the study of ExoU.Keywords
This publication has 18 references indexed in Scilit:
- Pseudomonas aeruginosaVirulence Analyzed in aDictyostelium discoideumHost SystemJournal of Bacteriology, 2002
- Type III protein secretion is associated with poor clinical outcomes in patients with ventilator-associated pneumonia caused by Pseudomonas aeruginosaCritical Care Medicine, 2002
- The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect the social amoeba Dictyostelium discoideumProceedings of the National Academy of Sciences, 2002
- Prevalence of type III secretion genes in clinical and environmental isolates of Pseudomonas aeruginosaMicrobiology, 2001
- Multiple Domains Are Required for the Toxic Activity of Pseudomonas aeruginosa ExoUJournal of Bacteriology, 2001
- Acquisition of Expression of the Pseudomonas aeruginosa ExoU Cytotoxin Leads to Increased Bacterial Virulence in a Murine Model of Acute Pneumonia and Systemic SpreadInfection and Immunity, 2000
- ExoU expression by Pseudomonas aeruginosa correlates with acute cytotoxicity and epithelial injuryMolecular Microbiology, 1997
- Identification of Pseudomonas aeruginosa genes required for epithelial cell injuryMolecular Microbiology, 1997
- Hospital-acquired pneumonia in adults: diagnosis, assessment of severity, initial antimicrobial therapy, and preventive strategies. A consensus statement, American Thoracic Society, November 1995.American Journal of Respiratory and Critical Care Medicine, 1996
- Molecular characterization of the patatin multigene family of potatoGene, 1988