Quantitative determination of tocopherols by high-speed liquid chromatography.
- 1 January 1975
- journal article
- research article
- Published by Center for Academic Publications Japan in Journal of Nutritional Science and Vitaminology
- Vol. 21 (3) , 183-188
- https://doi.org/10.3177/jnsv.21.183
Abstract
A high-speed liquid chromatographic method for the determination of alpha-, beta-, gamma- and delta-tocopherols using a spectrophotometer or a spectrofluorometer, is described. The best results were obtained using a 2.3 mm i.d. x 500 mm normal phase partition column, JASCO-PACK WC-03-500 and a spectrofluorometer as a specific detector. A mixture of diisopropyl ether and n-hexane (2:98) was used as the mobile phase. A flow rate was set at 0.8 ml/min. A mixture of pure alpha-, beta-, gamma- and delta-tocopherols or natural tocopherols in vegetable oils were separately determined by this method.Keywords
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