Analysis of Metal Ions by Sweeping via Dynamic Complexation and Cation-Selective Exhaustive Injection in Capillary Electrophoresis

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Abstract
To improve the detection sensitivity of metal ions in capillary zone electrophoresis (CZE), a novel method that combines complex formation and on-line sample preconcentration by sweeping was developed. Sweeping is defined as the picking and accumulating of analytes by a carrier in the background solution, with which they have considerable affinity. In this sweeping method, using ethylenediaminetetraacetic acid as carrier, dynamic complexation to form a UV-absorbing chelate and on-line preconcentration occur simultaneously during a run. The technique was validated in terms of the limit of detection, reproducibility, and sensitivity enhancement. Detection responses of some divalent metal ions, in terms of peak heights, were improved from 60- to 180-fold, relative to conventional CZE which employed precapillary complexation. The limits of detection were in the range of (1.8−23.4) × 10-8 M. This method was applied to the analysis of trace metal ions in factory wastewater. Furthermore, sweeping in conjunction with sample stacking accompanying electrokinetic injection, cation-selective exhaustive injection (CSEI−sweeping), was also examined. Up to 140 000-fold improvement in detector responses for some divalent and trivalent metal ions was realized by CSEI−sweeping. The limits of detection were in the range (2.4−25.2) × 10-11 M.

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